GlutaMAX Supplement (Life Technologies 35050-061) BSL-2 hood Precautions and Disclaimer This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. Preparation Instructions Medium by adding 5 mL of Penicillin-
Dysfunction of the retinal pigment epithelium (RPE) is implicated in numerous forms of retinal degeneration. The readily accessible environment of the eye makes it particularly suitable for the transplantation of RPE cells, which can now be derived from autologous induced pluripotent stem cells (iPSCs), to treat retinal degeneration. For RPE transplantation to become feasible in the clinic ...
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DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
mixture containing Glutamax-I (31331; Invitrogen), seeded onto the chip in 3 ml medium, and allowed to grow for 3 to 5 h. The distance between the lipid bilayer of the cells and silicon dioxide on chips was measured by FLIC microscopy (Lambacher and Fromherz 2002) with an average of 35 nm (n 28). RAT NEURONS. The chips were cleaned as for ...
Glutamax (2 mM), and antibiotic−antimycotic (1%) (Invitrogen). Each fetus was decapitated, and the whole brain was transferred to fresh ice cold dissecting medium. After isolation, the hippocampi were enzymatically digested at 37 °C for 10 min with papain (2 mg/mL, Worthington 3119), Hibernate-E without calcium (BrainBits) and 2
Glutamax Life Technologies 35050 IL1β Multiple Vendors IMDM Life Technologies 12440 MCSF Multiple Vendors PES Filter Unit, 0.2 µm . Multiple Vendors : Pipettes Multiple Vendors SFEM . Stem Cell Technologies . 09650 : Trypan Blue Gibco 15250 1 Ensure the automated cell counter is appropriately calibrated before use.